The Model CV1 Cuvette Module, shown to the left, interfaces with the Model S48D Illumination Source and the Model D48 Detector Module for fluorescence measurements of samples in standard cuvettes (10 x 10 mm path length).  Details of the Model CV-1 Cuvette Module are discussed elsewhere.

We now offer a host of accessories to further extend the capabilities of this versatile cuvette module. 

• A Clark electrode accessory to allow measurement of oxygen tension during measurements of fluorescence.
• A series of cuvette inserts to lower the minimum volume required for fluorescence measurement.
• A coverslip holder and cell perifusion system to measure fluorescence of cultured cells. 

Measure O₂ Consumption/Evolution During Fluorescence Measurements

Model CE-1 Oxygen Electrode Insert

With our special insert and Clark polarographic electrode, shown below, you can measure oxygen tension in your samples at the same time you are monitoring fluorescence!  The insert fits snugly into the top of a standard cuvette and it has an access port for easy addition of samples and reagents.  With the Model CE-1, simultaneous O₂ consumption and fluorescence measurements are easily accomplished.   

Best of all, you do not need a separate chart recorder or data acquisition system to record these data.  Either fluorometer controller provided with any C&L fluorometer system (PC-DAQ or RS-DAQ Controller) is capable of acquiring up to eight analog voltages along with fluorescence data.  Measuring oxygen tension in your samples is as easy as connecting the electrode to the oxygen electrode amplifier and connecting the output of the amplifier to one of the analog input channels of a C&L Instruments controller. 

Oxygen Electrode Insert shown with a microsyringe. click for a larger image

This system is ideal for monitoring O₂ consumption of cell suspensions or isolated mitochondria when these measurements are to be correlated with changes in other physiological parameters that can be measured with fluorescent probes (calcium, pH, membrane potential, etc.).  The top of the inset has an access port for easy addition of samples and reagents and this port aligns with a similar access port in the lid of the Model CV-1 Cuvette Module so that the lid does not have to be removed for the addition of samples with a microsyringe.  This feature prevents exposure of the cuvette to room light during an experiment.

click for a larger image

The oxygen amplifier used for these measurements is a product of Instech Laboratories (Model 203).  C&L instruments is proud to supply these amplifiers for use with our products. Instech's model 203 amplifier generates the potential that operates the O₂ electrode and converts the nanoamp electrode current into a voltage to measure pO₂.  The amplifier contains two independent channels, each with gain and zero offset controls.  A 3½ digit display is provided.  These signals can be sent to an analog input channel of either the Model PC-DAQ or RS-DAQ Controller for recording of pO₂ during fluorescence measurements.

Pricing Information

Specifications

Instech Model 203 Oxygen Amplifier

• Size: 10.5 (w) x 8.8 (l) x 4.5 (h) inches.
• Weight: 3.5 pounds.
• Power: 120 or 220 VAC (specify on order), 50/60 Hz.
• Signal output: 0-5 VDC.

Instech Model 125/05 Polarographic Oxygen Electrode

• Diameter: 0.125 inches.
• Typical current at ambient pO₂: 18 nAmps.
• Temperature coefficient: 4% / 1^oC.

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Cuvette Inserts Lower the Volume Requirement for Fluorescence Measurements

We manufacture several type of cuvette insert accessories that fit into the cuvette holder of the Model CV-1 Cuvette Module.  These inserts allow for fluorescence measurements using disposable glass test tubes or shell vials.  Contact us for a further details.

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Image of a cuvette insert designed to hold a 5 X 31 mm shell vial.  The vial contains a 50 microliter sample.  A US Nickel is shown for size comparison.  An insert for 6 X 50 mm disposable test tubes is also available.

 

 

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Coverslip Holder* Allows for Measurement of Fluorescence of Cultured Cells and Perifusion of Cultured Cells

To measure the fluorescence of cells in culture, you do not necessarily require a microscope.  Although a microscope is needed to measure fluorescence emitted from single cells, this cuvette insert allows for fluorescence measurements from a population of cultured cells.   Moreover, measurements can be made without having to detach the cells from their matrix support. 

CCH-1 Coverslip holder with syringe inserted.  click for larger image

If you can culture your cells of interest on a coverslip, you can place the coverslip directly into a standard cuvette using either our model CCH-1 or Model CCH-2 coverslip holder to measure cell fluorescence.   The Model CCH-1 holds a 11 x 22 mm coverslip in a standard 10 x 10 mm light path cuvette to permit fluorescence measurements of the cell layer.  

The Model CCH-2 is similar, but it also contains a tubing system to permit the exchange of the cuvette contents during the course of an experiment.  The tubing is 1/8 inch OD and can be connected to bulkhead connectors on the Model CV-1 Cuvette Module.  

Both models of coverslip holders have an access port on the top of the holder to permit the addition of reagents in to the cuvette without having to remove the coverslip from the media.  In addition, this access hole is conveniently aligned with a similar hole in the lid of the Model CV-1 Cuvette Module so reagents can be added to the cuvette in total darkness without removing the lid. The bottom of the coverslip is suspended above the bottom of the cuvette to permit use of a magnetic stir bar.

Model CCH-2 click for a larger image

This drawing shows the schematic of the Model CHH-2 Coverslip holder.  The coverslip is held in place by a small hex screw.  All wetted parts are either stainless steel or black PET (polyethylene terephthalate).  Changing coverslips is fast and easy.

click for a larger image

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For more information, you can download the specification sheet for the Model CCH-1 and CCH-2

Download CCH-1 and CCH-2 Specifications in PDF format (51KB)

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