This page provides brief descriptions of our products and accessories. To go directly to a specific item description, select its name from this list.  To see how these components can be configured to implement different types of fluorescence systems to meet your specific requirements, please see our Systems Overview page.

Fluorescence Software and Systems:

• C&L Dye Fluorometer, configured for microscopy or cuvettes

• FluorMeasure^TM Photometry Software

Detectors:

• Multiwavelength Photon-Counting Detector, Model D48

• Models DPC-BA and DPC-MA PMT Detectors

Photon Counting Systems:

• Digital Photon Counting System, Model DPC-1

• Digital Photon Counting System, Model DPC-2

Illuminators:

• Illumination Source, Model S48D

• Illumination Source, Model SPLL

Perfusion and Incubation Chambers:

• Vacu-Cell^TM Perfusion Chambers and Microscope Stage Adapters

Components and Accessories:

• Interference Filters

• Filter Wheel Assembly, Model MFW-4

• Cuvette Module, Model CV-1

• Accessories for Cuvettes

• Instrument Controller, Model PC-DAQ

• Total Internal Reflection Fluorescence Cells

• Fluorometer and Microscope Accessories

• KoolBlox for Eppendorf Tubes

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C&L Dye Fluorometer

The C&L Dye Fluorometer  is an innovative multiwavelength fluorometer designed specifically for use with readily available fluorescent dyes (shown here with an Olympus IX70 microscope and without a computer). The fluorometer comprises three main components: a system controller that resides in your PC, an illumination source, and a detection system. It interfaces with your Windows 98/2000 workstation and fluorescence microscope to make multiwavelength measurements of your micro specimens a straight forward procedure. When used without a fluorescence microscope, the fluorometer can be adapted for measurement of surface fluorescence or it can be connected to an optional cuvette module.  The light source of the fluorometer can be used together with a camera and our FluorImage^TM software to configure a powerful ratio imaging system.

• Multiwavelength selection for both excitation and emission. Up to 8 excitation and 8 emission wavelengths.
• Fast response time. Up to 8 excitation-emission wavelength pairs acquired at a rate of 1000 measurements per second.
• High spectral purity and high light throughput. This is achieved by utilizing our unique filter wheel design.
• Digital control of illumination intensity. Illumination intensity can be specified over a 50-fold range.
• Eight analog input channels. Simultaneous acquisition of data from other instruments. The data is captured in synchrony with the fluorescence measurements.
• Vibration-free operation. Light into and out of your microscope is via quartz light guides.
• Low cost of ownership. Contact us for a price list. You'll be glad you did. Complete multiwavelength excitation and multiwavelength emission fluorometer systems are available for under $20K. 

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Digital Photon Counting System
Model DPC-1

The Model DPC-1 Digital Photon Counting system includes an PCI PC card and software. It is a complete photon counting system that connects directly to photomultipliers having a TTL count output.  This photon counting system allows you to easily collect data directly from a photomultiplier into your computer.   The DPC-1 PCI PC card is easy to install and is completely Plug and Play compatible. The data acquisition and analysis software is Windows-based and is powerful, yet easy to use for fluorescence or other photometry photon counting measurements. The data can also be easily exported to other software programs. The system can be easily upgraded for use with C&L Instruments Multiwavelength Fluorescence Illuminators and Detectors.

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Digital Photon Counting System
Model DPC-2

The Model DPC-2 Digital Photon Counting system includes an PCI PC card, software and a breakout connector box.  It is similar to the DPC-1 (see above), in that it is a complete photon counting system that connects directly to photomultipliers having a TTL count output.  In addition, it is capable of collecting analog data simultaneously with the fluorescence data.  It can also be used just for analog data collection without use of a PMT.  The DPC-2 PCI PC card is easy to install and is completely Plug and Play compatible. The data acquisition and analysis software is Windows-based and is powerful, yet easy to use. The data can also be easily exported to other software programs. The system can be easily upgraded for use with C&L Instruments Multiwavelength Fluorescence Illuminators and Detectors.

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Model DPC-BA / DPC-MA PMT Detectors

The Model DPC-BA & DPC-MA Detector Modules are complete detector assemblies that contain a photon-counting photomultiplier tube in a compact enclosure with C-mount threads for easy microscope mounting. The Detector Module is designed for high-sensitivity photometry detection.  These detector modules can be purchased as part of a complete fluorometer system or separately to serve as a stand-alone detection system. They can also be added to fluorescence imaging systems to provide a faster and more sensitive means of fluorescence detection and quantitation.

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Interference Filters

We maintain a stock of premium quality interference filters.  Please check our page of Interference Filters for current availability and spectra.  The filters used in our instruments are custom designed to our high standards and are not available elsewhere as "stock" items.  These filters have very high wavelength specificity.  Typical transmission in the out-of-band region is > 6 OD.   If you are not familiar with the specifications of interference filters, you can read our tutorial. 

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Illumination Sources

We offer two different illumination sources. Both sources use a xenon arc lamp and a filter wheel for wavelength selection. They can be configured with a filter wheel containing either four, 1-inch diameter filters or eight, 1/2 diameter filters. The filter wheels are easy to balance, making high speed and vibration free operation possible, and are easy to replace. Light output is via a quartz light guide. These sources are ideal for high speed multicolor illumination. Either illuminator can also be used with a chopper wheel to provide a chopped, single wavelength or broad-band light source. The light guide interfaces either directly to a Cuvette Module or your fluorescence microscope using a microscope input accessory.

Illumination Source, Model S48D

The Model S48D is a computer controlled illumination source. This source can be controlled by either the Model PC-C or Model PC-DAQ PC Controller. The controller interacts with the Illumination Source through software residing on your personal computer. The software allows the illumination source to be controlled using several operational modes. All instrument settings can be stored, so making routine measurements becomes truly routine.

Illumination Source, Model SPLL



The Model SPLL is a stand-alone illumination source.  It does not require a computer for operation. This source produces a multiwavelength chopped light source, the frequency of which can be controlled either internally by the source or externally via a TTL-compatible input. An integral digital display indicates the operating speed of the source. The external input is available to synchronize the light source to an external device, such as a video camera or data acquisition system. The phase relation between the external input and chopped light can be adjusted over 351 degrees in 0.7 degree step increments in a manner that is independent of the chopped light frequency.

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Vacu-Cell^TM Incubation Chambers

C&L introduces the Vacu-Cell^TM line of cell incubation chambers. The unique design holds the coverslip or other surface by one side only, providing major advantages over cell chambers made by others. 

The coverslip or slide surface is held securely in place by application of a vacuum.  Dual o-rings isolate the vacuum from the area of the coverslip under study and securely hold the coverslip in place.  

Several configurations are available, including this laminar flow chamber, a round perfusion chamber and an open top design.

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Filter Wheel Assembly, Model MFW-4

This product houses a motorized filter wheel and proved a means for computer control of the excitation wavelength of your microscope.   It is show here connected between the lamp housing and the epi-illumination optics of a Nikon Diaphot illuminator.  With this inexpensive add-on to your microscope, you can quickly change the illumination wavelength to make multiwavelength measurements without having to change or move the dichroic cube.  This device is available in several formats and can be mounted to virtually any microscope or to a fiber optic probe.

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Detector Module, Model D48

This detection module incorporates a photomultiplier operating in the photon-counting mode and a filter wheel for selection of the detection wavelength. The detector module accepts light from a light guide which interfaces to to either the Model CV1 Cuvette Module or to your fluorescence microscope using a microscope output accessory. The Detector Module is controlled using the Model PC-DAQ PC Controller. The controller interacts with the Detector Module through software residing on your personal computer. Together, the software, PC-DAQ PC Controller and the Model D48 Detector Module function as a complete multiwavelength data acquisition system.

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Cuvette Module, Model CV-1

This module interfaces with the Model S48D Illumination Source and the Model D48 Detector Module for fluorescence measurements of samples in standard cuvettes (12.5 x 12.5 mm).  The cuvette holder is water-jacketed for temperature control using a external water bath (not supplied).  The Z height (height for the bottom of the cuvette to the center of the excitation beam) is 15 mm.  The Cuvette Module also contains a magnetic stir bar assembly.  Fluorescence emission is measured 90 degrees relative to the excitation beam.  Contact C&L Instruments if you require a different illumination and detection geometry.  Additional accessories are also available.  See our Accessory page for more details.

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Cuvette Accessories

We offer several cuvette accessories for use with our fluorescence systems.  The Model CE-1 holds a Clark electrode.  Use the CE-1 to monitor the Oxygen tension of the cuvette contents while measuring fluorescence.  

We also offer a variety of cuvette inserts that can be used to reduce the sample volume required to make fluorescence measurements.  

Show on the left is our popular Model CCH-2 coverslip holder.  This accessory holds a small coverslip within a standard cuvette.  It is very convenient for measuring the fluorescence of cultured cells and for exchange of the cuvette contents.  See our Accessory page for more details.  

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Instrument Controller, Model PC-DAQ

The Model PC-DAQ PC Controller is a PC card that is designed for the PCI bus of a PC-compatible computer. It allows the user to control both the Model S48D Illumination Source and the Model D48 Detector Module through software. Together, the Model S48D Illumination Source, Model D48 Detector Module and Model PC-DAQ PC Controller are the three main components of the C&L Dye Fluorometer. This PC card contains a complete photon counting system for multiwavelength fluorescence measurements, as well as an 8 channel analog data acquisition system. It can also be operated independently for acquisition of analog data. Easy-to-use software is available for card to unleash the full potential of its data acquisition and instrument control features.

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Components for TIRF

Total Internal Reflection Fluorescence (TIRF) is a powerful fluorescence technique that can be applied to a variety of analytical applications.  TIRF occurs when excitation energy is transmitted through a medium by total internal reflection.  This generates an thin evanescent energy layer that can excite fluorescent probes in close proximity to the surface of the medium.  We have TIRF cells containing a flow chamber for introduction of your samples to the TIRF surface.  With chemically-modified surfaces, this technique can be used for molecular screening and assessment of binding affinities.  TIRF is a powerful technique to assess protein-protein interacts.  Please contact us for more details.

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Fluorometer and Microscope Accessories

 

Olympus IX Series Input Adapter

Note: Additional accessories and microscope adapters are available on request. Please contact us.

• Four Position Filter Wheel (Part No. WN4)
  Filter wheel for mounting four 1-inch diameter filters.
• Eight Position Filter Wheel (Part No. WN8)
  Filter wheel for mounting eight 0.5-inch diameter filters.
• Arc Lamp (Part No. LN175)
  Replacement arc lamp for use with either Model S48D or Model SPLL Illumination Source.
• Olympus IX Series Input Adapter (Part No. MI-OIX)
  Microscope input interface for connecting either the Model S48D or Model SPLL Illumination Source to the Olympus IX series of microscopes.
• Nikon 300 Input Adapter (Part No. MI-N300)
  Microscope input interface for connecting either the Model S48D or Model SPLL Illumination Source to the Nikon 300 microscope.
• BK 05 Balancing Kit (Part No. BK05)
  Balancing Kit for Eight Position Filter Wheel.
• BK 10 Balancing Kit (Part No. BK10)
  Balancing Kit for Four Position Filter Wheel.

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KoolBlox

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