FluorMeasure^TM:
Software for Multiwavelength Photometry

FluorMeasure^TM is a software application for acquisition and analysis of fluorescence data that is the software suite used by the C&L Instruments Dye Fluorometer and Digital Photon Counting Systems (DPC-1 and DPC-2).  Our Dye Fluorometer system can attach to a fluorescence microscope for single cell measurements, to a cuvette assembly for solution fluorescence applications or to a bifurcated fiber optic probe for surface fluorescence measurements.  FluorMeasure^TM has powerful features that are simply just not available in any other fluorescence photometry system.  FluorMeasure^TM was truly designed from the ground up with the needs of the research scientist at the forefront.  The software suite consists of two applications; FluorMeasure^TM-Acquisition and FluorMeasure^TM-Analysis.   The separate FluorMeasure^TM Analysis software component allows you to analyze your data on more than one computer.

See our demonstration videos that you can download to view FluorMeasure^TM in action!

FluorMeasure^TM

FluorMeasure^TM-Acquisition is the data acquisition component application of FluorMeasure.  The software allows you to easily set up the Dye Fluorometer for any type of data acquisition sequence and save your data to a file. 

Key Features of FluorMeasure^TM:

	Allows measurement of up to eight separate excitation and eight emission wavelengths for multiwavelength and/or multi-probe fluorescence measurements.  Can be used for virtually any fluorescent probe.   The software is easily configured for ratio excitation and ratio emission probes (or both!).
	Fast data acquisition.  Software can acquire up to eight excitation-emission wavelength pairs in 8 milliseconds (1 KHertz/wavelength pair.  Fluorescence ratios (fura-2, indo-1, etc.) can be measured at 0.5 KHertz (500 ratios/second), continuously. A single wavelength pair (one excitation and emission wavelength) can be sampled at 1,000 points/second.
	Trigger data acquisition externally using a TTL pulse.  You can synchronize data acquisition with other systems, such as hardware for electrophysiology.
	Fluorescence displayed as counts or counts/second.
	Auto-increment file naming feature saves you time.
	Time lapse mode with automated shutters and data averaging capability.  Specify how often the specimen is to be sampled and just walk away.
	Monitor fluorescence from all acquisition wavelength channels in one or several program windows.
	Simultaneous acquisition of analog data can be monitored along with fluorescence data in one or several program windows.
	Monitor fluorescence ratios in real time.
	Computer control of excitation light intensity with 100 discrete intensity levels.
	Independent light intensity setting for each excitation - emission wavelength pair allows you to adjust measured intensities when measuring ratios and multi-probes.
	All systems setting can be saved in a configuration file for later use, making it easy to use in a multi-user environment.

Experiments in FluorMeasure^TM are easily set up using a multi-tabbed dialog box. You can set the instrument for single or multiwavelength measurements and save all the setting for later use. You have complete control of the filter wheels and shutters.  The excitation and emission shutters are controlled independently and can operate in several modes, from manual control to fully automated. 

Filter wheel support can also be disabled, which allows FluorMeasure^TM to be used for photon counting and analog data acquisition.

FluorMeasureTM Setup Sequencer page. Click on the image for a larger version

Excitation and emission wavelength filter changes can be programmed to sequence in the order the filters are placed in the filter wheels, or in any custom order to produce specific excitation - emission wavelength pairs. Specific excitation - emission pairs are programmed in a ring sequence that loops in the manner you specify during data acquisition. 

For a demonstration of how wavelength parameters are changed in FluorMeasure^TM, download the Changing the Wavelength Sequence video (3,157 KB).  See see our all our demonstration videos for more illustrations of FluorMeasure^TM.

Trigger set up in FluorMeasureTM.  Click on the image for a larger version

FluorMeasure^TM provides several options to trigger data acquisition.  Acquisition can be triggered internally with either a key stroke or mouse click, or externally with a TTL pulse.  Pre-trigger delays and post-acquisition re-arming delays makes it very easy to synchronize the FluorMeasure^TM data acquisition with other systems.  This is useful, for instance, if you are an electrophysiologist and want to synchronize your voltage clamp system to the acquisition of fluorescence data.  

Opening a data monitor window in FluorMeasureTM.  Click on the image for a larger version

Data from one or more fluorescence channels can be viewed in real time.  Each monitor window can be scaled independently and can be set up to monitor one or more fluorescence channels, one or more analog channels, or a fluorescence ratio.  The monitor window can be programmed to display the data in various color formats.  Zoom capabilities allow you to view your data at the resolution you prefer.  

See the video Acquiring Analog Data Simultaneously (4,033 KB).  This video illustrates how FluorMeasure^TM can be set up to acquire and display analog data in a synchronous fashion with fluorescence data using two data-monitoring windows.  See see our all our demonstration videos for more illustrations of FluorMeasure^TM.  

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