Large Window Rectangular Perfusion
Vacu-Cell^TM Chamber*

*Patent Pending

Large Window Rectangular Perfusion (Model RPC-LW)

Model RPC-LW, top and bottom  views, with coverslips attached

Model RPC-LW, bottom view   without coverslips attached

Model RPC-LW, cross section

The Large Window Rectangular Perfusion Chamber uses two rectangular coverslips to form a perfusion chamber.  Dual o-rings on the top of the chamber body and dual o-rings on the bottom securely hold two 48 x 65 mm coverslips in place.  Each coverslip is held in place using a separate vacuum port, so they can be used independently.

When the Model RPC-LW is used with two coverslips, a rectangular chamber is formed with the two coverslips spaced by approximately 6.1 mm (0.24 inches). The total volume contained in the observation window is 7.54 ml.  Inlet and outlet perfusion ports direct fluid through the chamber from the bottom edges. 

The perfusion ports can be configured for use with 1/16 OD tubing (as shown in drawings) or with hose barb connectors.  The vacuum ports connect to 1/16 inch ID tubing.  There are two vacuum ports supplied.  One is for the top coverslip and the other is for the bottom coverslip.  Either coverslip can be use separately.  In using only the bottom coverslip, an open well configuration can be established for access to the coverslip surface. 

A port for a 1/16 inch diameter temperature sensor is provided.  The sensor is led in place with a small thumb screw.  

Other Styles

We also manufacture other styles of Vacu-Cell^TM perfusion chambers for both circular and rectangular coverslips.  You can examine them on our product overview page.

Vacuum System

Vacuum Pump System (Model VCS-1)

We also offer a Vacuum Pump system for use with these perfusion chambers. The pump draws a vacuum of approximately -20 inches Hg and it fitted with a Luer-lock port for easy connectivity to standard tubing systems.  The Model VCS-1 vacuum pump assembly is approximately 8.4 (width) x 8.0 (depth) x 5.8 (height) inches in size.  These perfusion chambers also work well with other standard laboratory vacuum supplies.

Parylene-C Coated

All our Vacu-Cell^TM perfusion and incubation chambers that are made of black anodized aluminum are now also coated with Parylene-C.  The Parylene coating on the chamber is made to a uniform thickness of 0.5 mil (~12 microns).  This organic polymer coating makes the surface hard and completely bio-compatible.  It also exhibits a very low permeability to gasses.  This coating allows the chamber to be used for heat control (since aluminum conducts heat very well), but it effectively isolates the aluminum from your cells and media. You can read more about the remarkable properties of Parylene on our website. 

Stage Holders

Stage holders are available for many popular inverted microscopes.  This holder gently clamps the Vacu-Cell^TM on the stage of the microscope using two small thumb screws.  

Ordering Information, Chambers

Ordering Information, Stage Holders

(Specify microscope model when ordering)

Ordering Information, Coverslips

*No. 1, 0.13 - 0.16 mm thick
  No. 2, 0.19 - 0.23 mm thick

Download Product Specification Sheet

Pricing Information