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Large Window Rectangular Perfusion
Vacu-Cell
TM Chamber*

 

*Patent Pending

Large Window Rectangular Perfusion (Model RPC-LW)

Model RPC-LW Perfusion Chamber
Bottom of Large Window Perfusion Chamber

Model RPC-LW, top and bottom
 views, with coverslips attached

Bottom view without coverslips

Model RPC-LW, bottom view
  without coverslips attached

Sectioned view of Model VC-RPC-LW perfusion chamber

Model RPC-LW, cross section

The Large Window Rectangular Perfusion Chamber uses two rectangular coverslips to form a perfusion chamber.  Dual o-rings on the top of the chamber body and dual o-rings on the bottom securely hold two 48 x 65 mm coverslips in place.  Each coverslip is held in place using a separate vacuum port, so they can be used independently.

When the Model RPC-LW is used with two coverslips, a rectangular chamber is formed with the two coverslips spaced by approximately 6.1 mm (0.24 inches). The total volume contained in the observation window is 7.54 ml.  Inlet and outlet perfusion ports direct fluid through the chamber from the bottom edges. 

The perfusion ports can be configured for use with 1/16 OD tubing (as shown in drawings) or with hose barb connectors.  The vacuum ports connect to 1/16 inch ID tubing.  There are two vacuum ports supplied.  One is for the top coverslip and the other is for the bottom coverslip.  Either coverslip can be use separately.  In using only the bottom coverslip, an open well configuration can be established for access to the coverslip surface. 

A port for a 1/16 inch diameter temperature sensor is provided.  The sensor is led in place with a small thumb screw.  

Other Styles

We also manufacture other styles of Vacu-CellTM perfusion chambers for both circular and rectangular coverslips.  You can examine them on our product overview page.

Vacuum System

See Custom Specs

Vacuum Pump System (Model VCS-1)

Model VCS-1 Vacuum PumpWe also offer a Vacuum Pump system for use with these perfusion chambers. The pump draws a vacuum of approximately -20 inches Hg and it fitted with a Luer-lock port for easy connectivity to standard tubing systems.  The Model VCS-1 vacuum pump assembly is approximately 8.4 (width) x 8.0 (depth) x 5.8 (height) inches in size.  These perfusion chambers also work well with other standard laboratory vacuum supplies.

 

Parylene-C Coated

All our Vacu-CellTM perfusion and incubation chambers that are made of black anodized aluminum are now also coated with Parylene-C.  The Parylene coating on the chamber is made to a uniform thickness of 0.5 mil (~12 microns).  This organic polymer coating makes the surface hard and completely bio-compatible.  It also exhibits a very low permeability to gasses.  This coating allows the chamber to be used for heat control (since aluminum conducts heat very well), but it effectively isolates the aluminum from your cells and media. You can read more about the remarkable properties of Parylene on our website

 

Stage Holders

Stage holder, with Model VC-RPC-LWStage holders are available for many popular inverted microscopes.  This holder gently clamps the Vacu-CellTM on the stage of the microscope using two small thumb screws.  

 

 

Ordering Information, Chambers

Model Number Material
VC-RPC-LW-SS Stainless Steel
VC-RPC-LW-ORINGS Replacement O-Rings (5 Sets)

Ordering Information, Stage Holders

Model Number Microscope
VC-RPSH-OLY Olympus
VC-RPSH-NIK Nikon
VC-RPSH-ZEI Zeiss

(Specify microscope model when ordering)

Ordering Information, Coverslips

Model Number Description*
VC-48x60CS-1 48 x 60 mm, No.1, One Box

*No. 1, 0.13 - 0.16 mm thick
  No. 2, 0.19 - 0.23 mm thick

Download Product Specification Sheet

Pricing Information

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