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Thin Window Rectangular Perfusion |
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*Patent Pending
Thin Window Rectangular Perfusion (Model RPC-TW) The Thin Window rectangular perfusion chamber uses two rectangular coverslips to form a perfusion chamber. Two sets of dual o-rings on the bottom of the chamber body securely hold a 24 x 40 mm (upper) and a 48 x 65 mm (lower) coverslip in place. The open observation window measures 11.2 x 26.4 mm. The upper and lower coverslip are held in place using two separate vacuum ports, so they can be used independently. Inlet and outlet perfusion ports direct fluid flow from one end to the other end of the flow chamber between the two coverslips in a laminar flow pattern. When the Model RPC-TW is used with two coverslips, a rectangular laminar flow chamber is formed between the two coverslips. The space between the coverslips is 100 microns. The total volume contained between the two coverslips is 240 µl. Inlet and outlet perfusion ports direct fluid through the chamber. Either the upper or lower coverslip can be used without the other to fashion an open chamber configuration. The perfusion ports can be configured for use with 1/16 OD tubing (as shown in drawings) or with hose barb connectors. The vacuum ports connect to 1/16 inch ID tubing. There are two vacuum ports supplied. One is for the top coverslip and the other is for the bottom coverslip. Either coverslip can be used independent of the other. A port for a 1/16 inch diameter temperature sensor is provided. The sensor is led in place with a small thumb screw. NEW: View a 3D Interactive Model of this chamber! Other Styles We also manufacture other styles of Vacu-CellTM perfusion chambers for both circular and rectangular coverslips. You can examine them on our product overview page. Shear Stress Experiments Several of our Vacu-CellTM chambers designs are ideally suited for experiments where application of a controlled shear stress cells on a coverslip surface is desired. Details of how our chambers can be used for shear stress experiments are discussed in an Application Note. Several of our customers have critically evaluated these chambers for use in shear stress experiments and have given us testimonials about their performance. We invite you to read their comments.
Vacuum System
Vacuum Pump System (Model VCS-1)
Parylene-C Coated All our Vacu-CellTM perfusion and incubation chambers that are made of black anodized aluminum are now also coated with Parylene-C. The Parylene coating on the chamber is made to a uniform thickness of 0.5 mil (~12 microns). This organic polymer coating makes the surface hard and completely bio-compatible. It also exhibits a very low permeability to gasses. This coating allows the chamber to be used for heat control (since aluminum conducts heat very well), but it effectively isolates the aluminum from your cells and media. You can read more about the remarkable properties of Parylene on our website.
Stage holders are available for many popular inverted microscopes. These holders gently clamp the Vacu-CellTM on the stage of the microscope using a single retaining spring clip.
Ordering Information, Chambers
Ordering Information, Stage Holders
(Specify microscope model when ordering) Ordering Information, Coverslips
*No. 1, 0.13 - 0.16
mm thick Download Product Specification Sheet
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We also offer a Vacuum Pump system
for use with these perfusion chambers. The pump draws a vacuum of
approximately -20 inches Hg and it fitted with a Luer-lock port for easy
connectivity to standard tubing systems. The Model VCS-1 vacuum pump
assembly is approximately 8.4 (width) x 8.0 (depth) x 5.8 (height) inches in
size. These perfusion chambers also work well with other standard
laboratory vacuum supplies.
Stage Holders
