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Overview
Several of our customers have generously evaluated our laminar flow
chambers for use in shear stress experiments. Below are some of
their comments. If you would like to contribute comments to this
page, please send them to use using our
contact form.
- The Vacu-CellTM Round
Series Perfusion Chamber (Model VC-LFR-25) fabricated from clear acrylic
with a 0.1 mm gap distance between chamber bottom and glass slide was
tested in several experiments in our laboratory. Smooth muscle cells
were plated on 75 x 38 mm, 1 mm thick glass slides, which were sealed to
the perfusion chamber via the vacuum port. Cell culture media, at a flow
rate of 3.5 ml/min (supplied by a syringe pump), was introduced and
removed through the chamber’s entrance and exit ports, respectively. The
perfusion chamber with glass slide attached was mounted on an inverted
microscope and cell behavior in response to fluid flow shear stress was
examined for 30 minutes. Assuming fully-developed laminar flow between
infinite parallel plates, this flow rate, when coupled with the
appropriate dimensions of the perfusion chamber, exposed the cells to 25
dyn/cm2 shear stress. Experiments provided results similar to those
obtained using a different model perfusion chamber under similar
conditions (exposure to 25 dyn/cm2 shear stress). No difficulties were
encountered during experimental use of the perfusion chamber. The
chamber seems novel in design and practical for a range of applications
similar to that described here.
Zhong-Dong Shi, MS (graduate researcher)
Jeffrey S. Garanich, BS (graduate researcher)
John M. Tarbell, PhD (principal investigator)
City College of New York/CUNY Department of Biomedical Engineering
- "The chamber is
definitely the best we have tried with regard to ease of use"
Dr. Warwick Nesbit, Senior Research Officer, Monash University, Dept.
Medicine, Melbourne, Australia
To learn more about our unique Vacu-CellTM
design, start with our overview description of our
various models.

Categories
We offer Vacu-CellTM chambers in three different series
to suite various perfusion and microscopy requirements. Several
chambers can be used for either standard or inverted microscopes.
Follow
these links to pages that describe the various chambers available in these
three series, and the accessories used with the chambers.
Vacuum
System
Vacuum Pump System (Model
VCS-1)
We also offer a Vacuum Pump system
for use with these perfusion chambers. The pump draws a vacuum of
approximately -20 inches Hg and it fitted with a Luer-lock port for easy
connectivity to standard tubing systems. The Model VCS-1 vacuum pump
assembly is approximately 8.4 (width) x 8.0 (depth) x 5.8 (height) inches in
size. These perfusion chambers also work well with other standard
laboratory vacuum supplies.
Pricing
Information
Temperature
Controller
Temperature
Controller (Model TC-30)
We
provide a temperature controller designed to regulate the temperature of
our Vacu-CellTM chambers. The controller has several
features designed for precise, noise-free temperature control.
Literature
Download
our complete Vacu-CellTM product line specification literature (PDF
format).
A current price list is also
available.
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